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Enzymatic assays are typically performed using fluorescent and enzymatic substrates that are converted in the presence of an enzyme into fluorescent or non-fluorescent products that can be quantitatively detected. The substrates can include dyes that are chemically conjugated to or covalently bound to the analyte. The substrates are often labeled with a fluorescent dye that emits fluorescence in the presence of the enzyme.
A wide variety of different assays have been described that rely on the ability of enzymes to convert substrates into other analytes that can be detected. Examples of such assays include the following:
Enzymatic activity has been used to detect glucose in blood in order to facilitate the diagnosis of diabetes. Typically, conventional enzymatic assays involving blood have required complicated and time consuming procedures to effectuate the analysis.
In addition, advances in enzyme technology have made it possible to develop discrete elements that can be used in enzyme assays. These discrete elements have been designed to detect a specific analyte and to provide signals that are correlated to the presence or concentration of the analyte in a reliable manner. One such assay involves an enzyme-linked immunosorbent assay (ELISA) in which an antibody is covalently linked to the substrate of an enzyme, e.g., horseradish peroxidase (HRP), and a target analyte binds to the immobilized antibody. This ELISA is described in U.S. Pat. No. 4,327,111 (“the ‘111 patent”).
U.S. Pat. No. 4,327,111 describes an assay that requires a solution that includes reagents of two distinct types in different reaction vessels. One solution includes
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The Amazing Spider-Man, free and safe download. The Amazing Spider-Man latest version: Web-slinging fun through the streets of New York City.The present invention relates to motionless inductive loops that serve to transmit and receive pulses or oscillating signals that are picked up and transmitted along a cable and that serve to control the operation of a remote field device through inductive coupling.
The present invention particularly relates to the field of monitoring. This monitoring may be of a temperature, pressure, or chemical concentration level or may be of a power level, and may be carried out in a large industrial site or in a small area and may be of a stationary part such as a pipeline, a storage tank, a refuelling station, or a plant.
The monitoring may be carried out over large distances and the monitoring may be required by remote control through cables that have small cross sections.
The present invention also relates to such monitoring at small distances between remote field devices and remote power supplies or sources.
U.S. Pat. No. 3,583,113 discloses a pulse-detecting unit that is inductively coupled to a cable for powering and for receiving and transmitting pulses. The pulses are picked up and transmitted on the cable by a signal receiver and by a transmission coil having a plurality of turns. The number of turns of the transmission coil is a function of the distance of the monitoring of the pulses.
The U.S. Pat. No. 3,583,113 patent does not disclose the inductive coupling of the pulses to a remote control and monitoring device. Moreover, the present state of the art for inductive coupling in small distances requires the use of a tuned circuit in addition to the inductor and the cable.
U.S. Pat. No. 4,203,016 discloses an inductive coupling device including a magnetic core, a resonant tuned circuit, and a cable having a diameter of approximately 0.5 mm. The magnetic core is made of a material with a higher magnetic permeability than the cable in order to couple and transmit the pulses on the cable.
The U.S. Pat. No. 4,203,016 patent does not disclose a monitoring device in which the detector coil is a motionless coil with the same magnetomotive force as the control coil.Q:
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I need to execute the following command in a macruby script:
brutil list
I have tried the following